Puslinch is a township in south-central Ontario, Canada, in Wellington County, surrounding the south end of Guelph. The main source of production is agricultural, spring water bottling and mining. Aggregate mining has been dominant throughout the county. About half of the township is forested, and a conservation area lies to the southwest. Near the western edge of the township, just outside Cambridge, Ontario, is Puslinch Lake, the largest kettle lake in North America. It is part of the Guelph census metropolitan area.